This course is designed for a broad group of UCR student interested in life sciences. This course provides an introduction to the biochemical and molecular laboratory work performed in most labs today, and hence is a highly recommended course for all life science and medical track students.

The students will be introduced to the following themes:
• Blood
• Proteins
• Bacteria, hygiene and genetically modified organisms (GMOs)
• DNA
• Bioinformatics
These themes are characteristic or representative of clinical and biomedical research. Additional clinical diagnostic themes are added whenever there is an opportunity.

The concepts, principles and techniques used in this course regarding blood and blood proteins include a basic blood cell analysis (hemogram), protein electrophoresis, enzyme-linked immunosorbent assay (ELISA) and a patient case.

With respect to bacteria, a series of tests will be carried out to check hygiene and sterilisation procedures, bacterial species determination and the effect of antibiotics on bacterial growth. It also includes genetic modification of bacteria (Escherichia coli) using safe microbiological techniques.

Regarding DNA, human DNA isolation, gel electrophoresis, polymerase chain reaction (PCR) and restriction analysis will be performed. In addition students will generate a genetically modified organism (GMO). This will be followed up with a protein extraction as well as chromatographic purification of green fluorescent protein (GFP) from E.coli.

In addition to the experimental labs, one computer lab is planned to provide students the chance to become acquainted with some simulated (in silico) procedures, including data mining (DNA and protein data base searches).