This course is designed for a broad group of UCR students, e.g. life science students, science students and those interested in a medical track, to introduce them in the biochemical and molecular laboratory work of today, and hence is a highly recommended course for all life science majors and medical track students.
The students will be introduced to the following themes:
(1) Blood
(2) Proteins
(3) Bacteria, hygiene and Genetically Modified Organisms (GMOs)
(4) DNA
(5) Bioinformatics
These themes are characteristic or representative of clinical and biomedical research. Additional clinical diagnostic themes are added whenever there is an opportunity.

The concepts, principles and techniques used in this course regarding blood and blood proteins include a basic blood cell analysis (hemogram), protein electrophoresis, enzyme-linked immunosorbent assay (ELISA) and a patient case.

With respect to bacteria, a series of tests will be carried out to check hygiene and sterilization procedures, bacterial species determination and the effect of antibiotics on bacterial growth. It also includes genetic modification of bacteria (Escherichia coli) using safe microbiological techniques.

Regarding DNA, a human DNA isolation, gel electrophoresis, polymerase chain reaction (PCR) and restriction analysis will be performed. Also various DNA manipulations to make a GMO as well as chromatographic purification of green fluorescent protein (GFP) from E. coli will be done..

In addition to the experimental labs, one computer lab is planned to allow you the chance to become acquainted with some simulated (in silico) procedures, including data mining (DNA and protein database searches).
SCILABO201 Course Manual 2019-1.pdfSCILABO201 Course Manual 2019-1.pdf